Molecular characterization of multiple antibiotic-resistant Pseudomonas aeruginosa isolated from selected hospital fomites and hands of health care workers in Ondo, Nigeria

The study reported the molecular characterization, antibiotic susceptibility profile and nature of resistance genes in multiple resistant Pseudomonas aeruginosa isolated from selected hospital fomites hands health care workers Ondo, Nigeria. Various were swabbed for detection aeruginosa. Each sample was cultured separately on MacConkey Centrimide agar plates incubated 18-24h at 37oC. Pure isolates obtained using Analytical Profile Index (API) 20E kit. Kirby Bauer’s disc diffusion technique used to decide pure known antibiotics. Resistant 3 or more antibiotics determined by Polymerase Chain Reaction (PCR) appropriate primers. Two hundred Gram-negative bacterial recovered 480 swab-stick samples analyzed out which 54 aeruginosa.  All P. showed total augmentin, cefixime cefuroxime meanwhile 30 nitrofuratoin, gentamycin 11, ceftazidime 7, ofloxacin 2 ciprofloxacin 1. 31 (57.4%) three classes antibiotics.  Out 12 representative isolates, 6 harboured blaCTX-M (585 bp) gene not susceptible beta lactam while 4 7 Aminoglycoside (gentamycin) aac-3-iv (286 gene. In conclusion, different might be possible sources nosocomial infections.   Key words: aeruginosa, antibiotic-resistance, workers,